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Structured Review

Millipore polyclonal rabbit anti-glun2c
Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and <t>GluN2C</t> ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.
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1) Product Images from "Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation"

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

Journal: Translational Psychiatry

doi: 10.1038/s41398-020-01140-6

Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and GluN2C ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.
Figure Legend Snippet: Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and GluN2C ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.

Techniques Used: Expressing, Autoradiography, Western Blot

There was an environmental effect on the pGluA1 ( A ) expression in the BLA, but there were no effects of either environment or stress on the expression of GluA1( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as ## p < 0.01.
Figure Legend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the BLA, but there were no effects of either environment or stress on the expression of GluA1( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as ## p < 0.01.

Techniques Used: Expressing, Autoradiography, Western Blot

There was an environmental effect on the pGluA1 ( A ) expression in the FC, but there were no effects of either environment or stress on the expression of GluA1 ( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as # p < 0.05.
Figure Legend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the FC, but there were no effects of either environment or stress on the expression of GluA1 ( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as # p < 0.05.

Techniques Used: Expressing, Autoradiography, Western Blot



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Novus Biologicals rabbit polyclonal anti glun2c antibodies
FIGURE 7. Confirmation of the presence of GluN1, GluN2A, GluN2B, and <t>GluN2C</t> proteins in rat heart. Rat brain tissue homogenate was used as a control. Panel A shows the repre- sentative images in which the subunits were detected by immunoblotting. B, Changes in the GluN2B protein abun- dance in the heart with age of the animal. Actin was used as a loading control.
Rabbit Polyclonal Anti Glun2c Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore polyclonal rabbit anti-glun2c
Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and <t>GluN2C</t> ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.
Polyclonal Rabbit Anti Glun2c, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti-glun2c/product/Millipore
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-glun2c - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


FIGURE 7. Confirmation of the presence of GluN1, GluN2A, GluN2B, and GluN2C proteins in rat heart. Rat brain tissue homogenate was used as a control. Panel A shows the repre- sentative images in which the subunits were detected by immunoblotting. B, Changes in the GluN2B protein abun- dance in the heart with age of the animal. Actin was used as a loading control.

Journal: Journal of Cardiovascular Pharmacology

Article Title: Cardiac N-methyl d-aspartate Receptors as a Pharmacological Target

doi: 10.1097/fjc.0000000000000424

Figure Lengend Snippet: FIGURE 7. Confirmation of the presence of GluN1, GluN2A, GluN2B, and GluN2C proteins in rat heart. Rat brain tissue homogenate was used as a control. Panel A shows the repre- sentative images in which the subunits were detected by immunoblotting. B, Changes in the GluN2B protein abun- dance in the heart with age of the animal. Actin was used as a loading control.

Article Snippet: Staining was performed with the following primary antibodies: mouse monolconal anti-GluN1 antibody, and rabbit polyclonal anti-GluN2C antibodies (NB300-118 and NB300-107; Novus Biologicals), and rabbit polyclonal anti-GluN2A and mouse monoclonal anti-GluN2B antibodies (ab14596 and ab28373; Abcam).

Techniques: Control, Western Blot

Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and GluN2C ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: Stressed SC-housed animals showed higher expression of pGluA1 ( A ) and GluA1 ( B ) in the HC comparing to SC-housed, unstressed (only in B ), EE-housed, stressed and unstressed animals. EE-housed animals (both stressed and unstressed) presented the same expression levels of pGluA1 ( A ) and GluA1 ( B ) than SC-housed, unstressed animals. There were no differences in the GluN2B ( C ) and GluN2C ( D ) expression in both SC- and EE-housed animals (stressed or unstressed). There were no differences in the synaptophysin ( E ) and PSD-95 ( F ) expression in both SC- and EE-housed animals, regardless of the presence of stress. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A ; n = 6 for each group in B , D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test. Significance differences between groups are indicated as * p < 0.05 and ** p < 0.01.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot

There was an environmental effect on the pGluA1 ( A ) expression in the BLA, but there were no effects of either environment or stress on the expression of GluA1( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as ## p < 0.01.

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the BLA, but there were no effects of either environment or stress on the expression of GluA1( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as ## p < 0.01.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot

There was an environmental effect on the pGluA1 ( A ) expression in the FC, but there were no effects of either environment or stress on the expression of GluA1 ( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as # p < 0.05.

Journal: Translational Psychiatry

Article Title: Environmental enrichment prevents the late effect of acute stress-induced fear extinction deficit: the role of hippocampal AMPA-GluA1 phosphorylation

doi: 10.1038/s41398-020-01140-6

Figure Lengend Snippet: There was an environmental effect on the pGluA1 ( A ) expression in the FC, but there were no effects of either environment or stress on the expression of GluA1 ( B ), GluN2B ( C ), GluN2C ( D ), synaptophysin ( E ), and PSD-95 ( F ) in the same brain area. Representative autoradiography of western blot ( G ). Results are represented as mean ± SEM ( n = 5–6 animals per group in A and B ; n = 6 for each group in D – F ; n = 3 for each group in C ). Two-way ANOVA followed by Tukey’s multiple comparisons test (no differences were detected between the experimental groups). ANOVA significant main effect is indicated as # p < 0.05.

Article Snippet: Blots were blocked with 5% bovine serum albumin (BSA) diluted in TBS-T buffer (50 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.5) for 1 h at room temperature, and subsequently incubated overnight at 4 °C with specific primary antibodies: polyclonal rabbit anti-GluA1 (AB1504, dilution 1:2000; EMD Millipore Corporation), polyclonal rabbit anti-Ser845phospho-GluA1 (AB5849, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2B (#454582, dilution 1:1000; EMD Millipore Corporation), polyclonal rabbit anti-GluN2C (#454584, dilution 1:1000; EMD Millipore Corporation), monoclonal mouse anti-synaptophysin (AB8049, dilution of 1:20,000; Sigma-Aldrich Corporation), and monoclonal mouse anti-PSD-95 (AB99099, dilution of 1:20,000; Abcam).

Techniques: Expressing, Autoradiography, Western Blot